how to culture a cell line of "fibroblast"
Posted 20 May 2002 - 06:42 AM
can sombody tell me? thank you very much!!
Posted 22 May 2002 - 06:44 AM
Posted 23 May 2002 - 01:23 AM
Posted 25 May 2002 - 01:29 AM
if u want to use Fibroblast cell line, try NIH 3T3 cells but u can also use skin for primary cells culture.
Posted 08 June 2002 - 06:48 AM
Posted 09 June 2002 - 06:43 AM
You have to passage the cells when they become too close. The pieces of tissue can be discarded with the first passage.
Careful with skin samples! Ceratinocytes have also the ability to outgrow. Put the pieces in the flask so that the epidermis has no contact to the plastic. But even if ceratinocytes grow in your first culture usually you will remove them with the passages, since they get not detached within 4-6 minutes like fibroblasts.
Posted 01 July 2010 - 05:49 AM
i have tried to grow fibroblast from gingiva, but it was not working.
could you help me with more details on the media because i find that certain media contain different ingredients.
hope you can help me..my email : firstname.lastname@example.org