Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Nested DNA deletions - any experience?


  • Please log in to reply
No replies to this topic

#1 angelawu

angelawu

    member

  • Active Members
  • Pip
  • 12 posts
0
Neutral

Posted 22 March 2011 - 06:59 PM

HI All,

I'm wondering if anyone here has done nested DNA deletion cloning using Exonuclease III followed by Mung bead or S1 nuclease? This looks like a very intriguing technique, but I'm wondering if the Exo-3 digestion can really be well controlled such that it won't overdigest?

I guess I'm looking for if someone has had good or bad experiences with this technique it would be great to know (e.g. "I've tried it before, doesn't work well at all, don't waste your time" or "It's a great technique, very consistent", etc).

Thank you in advance!

Angela




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.