Posted 22 March 2011 - 10:22 AM
Posted 22 March 2011 - 11:44 AM
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
Posted 28 March 2011 - 10:08 AM
For my own practice, I do made master mix and store in -20 almost like you, but without the primers and taq. I had store it more than 3 months, still working.
Thanks ! My concern is that I have already added the primers! So not sure if they will form dimers and then not give a good result.
Posted 28 March 2011 - 12:52 PM
Posted 28 March 2011 - 01:03 PM
Should be fine. You denature things as a first step, so any annealing will be gone very rapidly.
Thanks Phage 434... your explanation makes good sense. Have just set up the PCR. Will definitely post the results ... keeping my fingers crossed as of now!