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Antigen coating in ELISA


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#1 kalindi

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Posted 22 March 2011 - 05:19 AM

I have a very basic question

how antigen coating is done inn ELISA???

what should be the concentration of antigen per well?

n how can you calculate that??

#2 lizh

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Posted 22 March 2011 - 07:59 PM

I have a very basic question

how antigen coating is done inn ELISA???

what should be the concentration of antigen per well?

n how can you calculate that??


For starters, u can try 1ug/ml concentration for antigen coating. Coating concentration really depends on what is the antigen, protein antigen generally require smaller amounts compared to say, lipid or carbohydrate antigens. Try with PBS to dissolve the antigen to 1ug/ml concentration.

The amount to coat for each well can be 50ul per well.

All the best!

LizH

#3 PDGF

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Posted 30 March 2011 - 06:17 PM

Buy the plates. Antigenix America sells some very good ones for a reasonable price.




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