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Dry protein gels without cracking


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5 replies to this topic

#1 sarah87

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Posted 22 March 2011 - 02:01 AM

Hi all,

I am fairly new to carrying out SDS-PAGE. I am a masters student using tricine sds page for my project. I want to dry the gels and keep them in my lab book.
Currently we soak the gel in 20% ethanol, 10% glycerol solution for 30min then place the gel between cellofane sheets.

Does anyone have another method/technique that won't cause cracks in the gels?? I know to get rid of the air bubbles etc, but my gels looked perfect under the cellofane, and then they cracked a day later. Any methods not requiring the purchase of expensive equipment??


Thanks :)

Sarah

#2 pito

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Posted 22 March 2011 - 02:20 AM

There are some protocols out there.
Have you checked those yet?
I think you are not letting it rest, dry long enough.


http://www.geba.org/...on Handbook.pdf
http://www.invitroge...gel-drying.html


and check this old topic on this forum:
http://www.protocol-...osts/40521.html
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.

#3 K.B.

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Posted 22 March 2011 - 06:01 AM

I never tried drying gel using only cellophane. I had frame like this:
Posted Image
I haven't paid a penny, they were made by my friend, cut (manually) from two pieces of acrylic glass.

#4 sarah87

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Posted 24 March 2011 - 08:20 PM

I never tried drying gel using only cellophane. I had frame like this:
Posted Image
I haven't paid a penny, they were made by my friend, cut (manually) from two pieces of acrylic glass.

Thanks for your replies!

I am getting better. No one in my lab told me that i needed to soak the cellofane :rolleyes: haha
So that must have been a big part of the problem. And i forgot to mention i have the apparatus above :)

cheers

#5 pito

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Posted 25 March 2011 - 11:25 AM

You didnt soak the cellophane? Uh? How did you dry it then? You just left it there to dry without doing anything? lol;)
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.

#6 sarah87

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Posted 26 March 2011 - 12:08 AM

You didnt soak the cellophane? Uh? How did you dry it then? You just left it there to dry without doing anything? lol;)

Oh soaked the gel in drying solution for 30min, and put some drying solution on the cellophane...but didnt soak the cellofane per say...no one showed me haha




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