The Good Antibodies for Western Blots Thread
Posted 21 March 2011 - 10:04 AM
I was recently testing a couple of GFP antibodies for mouse cardiac tissue. We have long used Abcam 290 but it was giving me a nonspecific band directly above the GFP band. So we tried another, Vector Labs BA-0702, which worked wonderfully well. I've attached a picture so you can see the difference. The picture isn't perfect but you can clearly see that the Abcam (top) has a nonspecific band, whereas the Vector Labs version (bottom) is clean.
So... if anyone would like to share decent iNOS or tubulin antibodies for use on mouse protein, I'd be much obliged. It sounds like tubulin is the way to go for a housekeeping protein if you're looking at ischemic tissue, so I need to check one out.
Posted 22 March 2011 - 10:18 PM
GFP from Roche. It is a mouse one as well. The mixture of two clones, 7.1 and 13.1.
"This is SPARTA!"
"I´m the goddamn batman"
Posted 13 April 2011 - 01:12 PM
That said, I was hoping someone might be able to suggest antibodies for any of the following.
beta1 or beta2 adrenergic receptor proteins
Thanks to anyone who can help.
Posted 09 April 2013 - 12:54 PM
I'd like to know what antibodies work for people as well.
GPCR antibodies especially. Angiotensin antibodies specifically. Anyone have any suggestions for these?