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Why would you monitor spectrin levels?


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#1 11235

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Posted 21 March 2011 - 09:41 AM

Why would someone want to monitor actin and spectrin levels? through Western Blotting? What would it show if they used actin and spectrin antibodies?

#2 bob1

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Posted 21 March 2011 - 02:30 PM

What do you know about actin and/or spectrin in the cell? What are their expressions like (levels etc.)?

#3 11235

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Posted 01 April 2011 - 01:15 PM

the levels are generally the same. But, I was wondering what actin and spectrin have to do with synaptic plasticity in layman's terms

#4 bob1

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Posted 03 April 2011 - 03:51 PM

Bugger all...

They are standard proteins used to ensure that you have loaded similar amounts of protein per sample.

#5 11235

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Posted 03 April 2011 - 04:54 PM

so, spectrin degradation and actin polymerization doesn't apply?

#6 bob1

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Posted 05 April 2011 - 04:33 PM

Possibly, I don't know about synaptic plasticity. However, what I said above is the most common reason people use actin and other common structural components in western blots.

#7 11235

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Posted 06 April 2011 - 11:58 PM

ok thank you so much!




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