Why would someone want to monitor actin and spectrin levels? through Western Blotting? What would it show if they used actin and spectrin antibodies?
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#2
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Posted 21 March 2011 - 02:30 PM
What do you know about actin and/or spectrin in the cell? What are their expressions like (levels etc.)?
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Posted 01 April 2011 - 01:15 PM
the levels are generally the same. But, I was wondering what actin and spectrin have to do with synaptic plasticity in layman's terms
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Posted 03 April 2011 - 03:51 PM
Bugger all...
They are standard proteins used to ensure that you have loaded similar amounts of protein per sample.
They are standard proteins used to ensure that you have loaded similar amounts of protein per sample.
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Posted 03 April 2011 - 04:54 PM
so, spectrin degradation and actin polymerization doesn't apply?
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Posted 05 April 2011 - 04:33 PM
Possibly, I don't know about synaptic plasticity. However, what I said above is the most common reason people use actin and other common structural components in western blots.
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Posted 06 April 2011 - 11:58 PM
ok thank you so much!
