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HEp2 cell fixation

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#1 orchidman2



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Posted 20 March 2011 - 06:27 PM

I have always used methanol at -20C for HEp2 cell fixation when grown on slides, but it doesn't give as good activity as acetone. Acetone does not retain nuclear morphology for mitotic cells. Does anyone have a fixative or process that will allow me to maintain mitotic cell morphology and high reactivity for IFA/ANA testing.

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