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Total Cell protein


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#1 shwari1

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Posted 20 March 2011 - 04:43 PM

Hi!

I'm a bit of newbie when it comes to protein purification. I am currently doing cell culture and purification.
My protein of interest is lac repressor (LacI). I need to find the total cell protein after cell culture.
Can anyone tell me how this is done? Thanks

#2 bob1

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Posted 21 March 2011 - 02:01 PM

How do you mean "find"? Do you want to know how much protein is present in a lysed solution? IF so, there are a range of quantitative methods you can use. The more common of which are known as the Bradford assay and the BCA assay.




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