cloning of race products
Posted 20 March 2011 - 06:45 AM
I am trying to perform 3' race and am using invitrogen kit. I got amplification using my gsp and kit supplied UAP . I am not sure now whether I can clone it into ta vector. The presence of uracil is going to affect or not.
Posted 20 March 2011 - 10:08 AM
I don't understand what uracil you're talking about, uracil is in the RNA, and you had to RT the transcript and amplify it, unless it's a kit with dUTP (to prevent cross-contamination), there should be no uracil. And if that is the case I don't think it would interfere with cloning, but E.coli cells (says Wikipedia)contain UNG that cuts off product with dUTP in it.
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