Hello everyone! I would like to seek advice on which bLAST PROGRAM I SHOULD USE IN THE FOLLOWING TWO CASES.
1. iF I WANT TO IDENTIFY PROTEIN-CODING GENES IN A NEWLY SEQUENCED GENOME WITH THE HELP OF THE uNIPROT DATABASE, SHOULD I USE tblastx?
2. iF HAVE THE AMINO ACID SEQUENCE OF A PROTEIN FROM e COLI AND I WANT TO IDENTIFY RELATED PROTEIN IN ANOTHER BACTERIAL SPECIES BUT A bLASTp SEARCH GAVE NO SIGNIFICANT MATCH, SHOULD i USE tblastn INSTAED?
tHX EVERYONE!
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