I want to precipitate the protein twice with amoinum sulfate. First 45 % and then 100 %. When I will do 45% initial amount of ammonium sulfate is 0. I will add the required am. sulfate and then centrifuge the solution and dicard the pellet and take the supernatent. This supertatent I want to precipitate to 100 %. For this step initail ceoncetration of am. sulfate is 0 or 45 % to count the amount of am. sulfate for second 100 % precipitation.
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#2
pDNA
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Posted 19 March 2011 - 10:22 PM
for the second step the starting concentration is 45%.
Regards,
p
Regards,
p
samita, on 19 March 2011 - 12:30 PM, said:
I want to precipitate the protein twice with amoinum sulfate. First 45 % and then 100 %. When I will do 45% initial amount of ammonium sulfate is 0. I will add the required am. sulfate and then centrifuge the solution and dicard the pellet and take the supernatent. This supertatent I want to precipitate to 100 %. For this step initail ceoncetration of am. sulfate is 0 or 45 % to count the amount of am. sulfate for second 100 % precipitation.
#3
samita
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Posted 20 March 2011 - 08:35 AM
Thanks
pDNA, on 19 March 2011 - 10:22 PM, said:
for the second step the starting concentration is 45%.
Regards,
p
Regards,
p
samita, on 19 March 2011 - 12:30 PM, said:
I want to precipitate the protein twice with amoinum sulfate. First 45 % and then 100 %. When I will do 45% initial amount of ammonium sulfate is 0. I will add the required am. sulfate and then centrifuge the solution and dicard the pellet and take the supernatent. This supertatent I want to precipitate to 100 %. For this step initail ceoncetration of am. sulfate is 0 or 45 % to count the amount of am. sulfate for second 100 % precipitation.
