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ammonium precipitation of Proteins


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#1 samita

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Posted 19 March 2011 - 12:30 PM

I want to precipitate the protein twice with amoinum sulfate. First 45 % and then 100 %. When I will do 45% initial amount of ammonium sulfate is 0. I will add the required am. sulfate and then centrifuge the solution and dicard the pellet and take the supernatent. This supertatent I want to precipitate to 100 %. For this step initail ceoncetration of am. sulfate is 0 or 45 % to count the amount of am. sulfate for second 100 % precipitation.

#2 pDNA

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Posted 19 March 2011 - 10:22 PM

for the second step the starting concentration is 45%.

Regards,
p

I want to precipitate the protein twice with amoinum sulfate. First 45 % and then 100 %. When I will do 45% initial amount of ammonium sulfate is 0. I will add the required am. sulfate and then centrifuge the solution and dicard the pellet and take the supernatent. This supertatent I want to precipitate to 100 %. For this step initail ceoncetration of am. sulfate is 0 or 45 % to count the amount of am. sulfate for second 100 % precipitation.



#3 samita

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Posted 20 March 2011 - 08:35 AM

Thanks

for the second step the starting concentration is 45%.

Regards,
p


I want to precipitate the protein twice with amoinum sulfate. First 45 % and then 100 %. When I will do 45% initial amount of ammonium sulfate is 0. I will add the required am. sulfate and then centrifuge the solution and dicard the pellet and take the supernatent. This supertatent I want to precipitate to 100 %. For this step initail ceoncetration of am. sulfate is 0 or 45 % to count the amount of am. sulfate for second 100 % precipitation.






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