Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Cell Line Establishment


  • Please log in to reply
1 reply to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 15 November 2000 - 10:00 PM

I am trying to establish primary cell lines from head and neck tumors. I seem to be able to grow them, but I can't seem to be able to passage them without the normal fibroblasts from overgrowing the culture and ruining my chances of growing the head and neck tumor.

#2 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 29 November 2000 - 10:00 PM

I suggest that you try to avoid using trypsin for passaging the cells. Use 2mM EDTA [in saline] instead. This way, fibroblasts will not be removed from the plastic dish and will not be passaged to the next plate.

[url="http://www.tau.ac.il/medicine/NLGIP/nlgip.htm"][url]http://www.tau.ac.il/medicine/NLGIP/nlgip.htm[/url][/url]





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.