Edited by precip_man, 17 March 2011 - 12:18 PM.
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Posted 17 March 2011 - 12:17 PM
My protein only has two tyrosines and no tryp's. IS the absorbance at 280 still accurate even though there are 0 tryps? The extinction coef i get from Expasy protparam is around 4470 for a 96 residue protein. What is the error associated to this absorbance? I have used bradford, but i feel there is more protein than the bradford is telling me, so maybe that method isnt as reliable. Thanks