I am trying to make a G418 killing curve for Hela S3 cells (untransfected). I seed the cells 8000/well in 96-well dish and after 24 hrs, add the antibiotic from 50 ug/ml to 2 mg/ml. It doesn't kill the cells. Recommended concentration for selection is generally 800 ug/ml for Helas. I was wondering if anyone working with G418 observed anything like. I'll be glad to hear your suggestions.
Edited by laraka, 17 March 2011 - 11:35 AM.