3'UTR luciferase assay with 5 miRNA target sites
Posted 17 March 2011 - 08:00 AM
Posted 18 March 2011 - 10:50 PM
Posted 24 March 2011 - 06:54 AM
I would like to design a 3'UTR with 5 miRNA target sites for a luciferase assay. I need to know how close the target sites can be. Does anyone have experience with this?
My favorite miRNA supplier did a similar custom job for me, but the journal wanted more definitive 1:1 proof first. I'd start with these: "http://www.abmgood.com/miRNA(microRNA)/3'UTR-miRNA-Reporters.html"