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3'UTR luciferase assay with 5 miRNA target sites


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#1 peebee

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Posted 17 March 2011 - 08:00 AM

I would like to design a 3'UTR with 5 miRNA target sites for a luciferase assay. I need to know how close the target sites can be. Does anyone have experience with this?

Thanks!

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#2 pcrman

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Posted 18 March 2011 - 10:50 PM

I guess there is no definite rule regarding how close the targets should be. You can put them next to each other or add a few based in between. Since you have 5 targets, and you are going to synthesize oligos to create the insert, there is no much room for you to add many extra bases between targets.

#3 Earnest

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Posted 24 March 2011 - 06:54 AM

I would like to design a 3'UTR with 5 miRNA target sites for a luciferase assay. I need to know how close the target sites can be. Does anyone have experience with this?

Thanks!

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My favorite miRNA supplier did a similar custom job for me, but the journal wanted more definitive 1:1 proof first. I'd start with these: "http://www.abmgood.com/miRNA(microRNA)/3'UTR-miRNA-Reporters.html"




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