How many cells do you have in a confluent T 75 flask?
Posted 17 March 2011 - 05:44 AM
In our lab we isolate cells from a cancer tissue, now in passage 5, but when I count the cells with a hemocytometer I count about half million of total cells in a CONFLUENT T 75! I thing is few cells (sorry for my english). When I saw the cells in a microscope, they are big , could be it a explication for the poor count?
how many cells did you count in your labs?
thanks a lot!
Posted 17 March 2011 - 01:02 PM
Posted 10 May 2014 - 11:59 AM
i had the same problem with melanoma cell line the cells are clamping and leading to error in counting
we used from EDTA 0.02% to solve this problem.