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[Luthien]

Hello everybody!
In our lab we isolate cells from a cancer tissue, now in passage 5, but when I count the cells with a hemocytometer I count about half million of total cells in a CONFLUENT T 75! I thing is few cells (sorry for my english). When I saw the cells in a microscope, they are big , could be  it a explication for the poor count?

how many cells did you count in your labs?

thanks a lot!

[bob1]

That is a fairly low number of cells.  For a standard size cell, you should get around 1x10⁷ cells.  What sort of cells are you growing out as yours seem extremely large which makes me think that they are probably entering senescence and won't grow any further?