Hi..
Can anyone please enlighten me with the function of internal controls used for nucleic acid purification/isolation.
Thank you..
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4 replies to this topic
#2
Adrian K
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Posted 17 March 2011 - 06:50 AM
In simple, to tell that your amplification(PCR) is working although there is negative result(no amplicons, except the internal control).
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
#3
Samantha30
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Posted 18 March 2011 - 09:45 PM
Thank you soo much.
Also can you please help me with the difference between Carrier RNA and Carrier DNA and also there function (or sequence if you can help me with) .
Actually I have been using some kits for DNA isolation and purification, where in one kit, they are recommending the use of carrier DNA and in the other, carrier RNA.
Both are for DNA isolation, then why is this difference there?
Also can you please help me with the difference between Carrier RNA and Carrier DNA and also there function (or sequence if you can help me with) .
Actually I have been using some kits for DNA isolation and purification, where in one kit, they are recommending the use of carrier DNA and in the other, carrier RNA.
Both are for DNA isolation, then why is this difference there?
#4
Adrian K
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Posted 19 March 2011 - 09:32 AM
what kit is that? Brand, model? Catalog?
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
#5
Samantha30
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Posted 20 March 2011 - 09:07 PM
Its Qiagen's kit for DNA purification.
QIAamp DNA Mini kit where they are recommending the use of Carrier DNA when the sample is low copy and
another kit is QIAamp DNA Micro Kit where they are recommending the use of Carrier RNA.
QIAamp DNA Mini kit where they are recommending the use of Carrier DNA when the sample is low copy and
another kit is QIAamp DNA Micro Kit where they are recommending the use of Carrier RNA.
