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can i use trypsin to collect cell sample for 2d gel analysis?


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3 replies to this topic

#1 yimao

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Posted 16 March 2011 - 01:38 AM

can i use trypsin to collect cell line sample from plate for 2d gel proteomic analysis? would that influence much about the result of experiment?

or should i only use a "rubber police"?

thx~

Edited by yimao, 16 March 2011 - 01:44 AM.


#2 mdfenko

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Posted 16 March 2011 - 11:28 AM

you can use trypsin as long as you wash the cells thoroughly before you lyse them.
talent does what it can
genius does what it must
i do what i get paid to do

#3 yimao

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Posted 16 March 2011 - 07:26 PM

you can use trypsin as long as you wash the cells thoroughly before you lyse them.


u mean after collect the cell by trypsin, right?

3 time by Wash Buffer good enough? :)

Edited by yimao, 16 March 2011 - 07:27 PM.


#4 mdfenko

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Posted 17 March 2011 - 05:59 AM

u mean after collect the cell by trypsin, right?

yes

3 time by Wash Buffer good enough? :)


if you drain the pellets well
talent does what it can
genius does what it must
i do what i get paid to do




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