3 replies to this topic

[yimao]

can i use trypsin to collect cell line sample from plate for 2d gel proteomic analysis? would that influence much about the result of experiment?

or should i only use a "rubber police"?

thx~

Edited by yimao, 16 March 2011 - 01:44 AM.

[mdfenko]

you can use trypsin as long as you wash the cells thoroughly before you lyse them.

[yimao]

you can use trypsin as long as you wash the cells thoroughly before you lyse them.

u mean after collect the cell by trypsin, right?

3 time by Wash Buffer good enough?

Edited by yimao, 16 March 2011 - 07:27 PM.

[mdfenko]

u mean after collect the cell by trypsin, right?

yes

3 time by Wash Buffer good enough?

if you drain the pellets well