or should i only use a "rubber police"?
thx~
Edited by yimao, 16 March 2011 - 01:44 AM.
Submit your paper to J Biol Methods today!
can i use trypsin to collect cell sample for 2d gel analysis?
Started by yimao, Mar 16 2011 01:38 AM
3 replies to this topic
#1
yimao
-
- Active Members
-
- 9 posts
member
0
Neutral
Neutral
Posted 16 March 2011 - 01:38 AM
can i use trypsin to collect cell line sample from plate for 2d gel proteomic analysis? would that influence much about the result of experiment?
or should i only use a "rubber police"?
thx~
or should i only use a "rubber police"?
thx~
#2
mdfenko
-
- Active Members
-
- 2,709 posts
an elder
123
Excellent
Excellent
Posted 16 March 2011 - 11:28 AM
you can use trypsin as long as you wash the cells thoroughly before you lyse them.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
yimao
-
- Active Members
-
- 9 posts
member
0
Neutral
Neutral
Posted 16 March 2011 - 07:26 PM
you can use trypsin as long as you wash the cells thoroughly before you lyse them.
u mean after collect the cell by trypsin, right?
3 time by Wash Buffer good enough?
Edited by yimao, 16 March 2011 - 07:27 PM.
#4
mdfenko
-
- Active Members
-
- 2,709 posts
an elder
123
Excellent
Excellent
Posted 17 March 2011 - 05:59 AM
yesu mean after collect the cell by trypsin, right?
3 time by Wash Buffer good enough?
if you drain the pellets well
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
