Subculturing primary Keratinocytes
Posted 05 June 2001 - 09:00 PM
I'm trying to isolate and culture Keratinocytes from human Skin. Isolation and culturing works well, but I've got problems subculturing them. I'm working with a Trypsin/EDTA solution in PBS without Calcium an Magnesium. Sometimes subculturing works and sometimes not. I have also problems with Fibroblasts overgrowing my Keratinocytes.I use a serumfree Medium caled EpiLife from Cascade Inc.
If somebody has experience with Keratinocyte Cultures, contact me.
Posted 08 June 2001 - 09:00 PM
I hope this helps.
Posted 25 December 2004 - 11:03 PM