Round hole in monolayer
Posted 15 March 2011 - 06:52 PM
Another T84 question from me:)
I've been growing these cells for some time, and the latest flask I've seeded is close to being confluent, but there is a large round hole in the monolayer (visible to the naked eye), where no cells are growing- they also seem to be bunching up around the edge of the hole. Is it likely to be a problem with the plastic surface so the cells can't grow there or could it be a virus infection??
Posted 16 March 2011 - 04:15 PM
Posted 20 March 2012 - 01:26 PM
I am facing this same problem, but instead I am working with HepG2 cells. Does anyone know the reason for such thing? Thnks
Posted 20 March 2012 - 06:11 PM