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urgent!!! weird Growth in cell line


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8 replies to this topic

#1 Sathyen

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Posted 15 March 2011 - 07:20 AM

hi guys we have recently had fungal contamination in our lab however it was thoroughly cleaned and all flasks changed etc etc.....today wehn i was doing regular viewing of my cells i saw something blurry. when i fine focused into the blur i observerd this.

http://img233.images...dsc00545cn.jpg/

http://img826.images.../dsc00538e.jpg/

http://img135.images.../dsc00541c.jpg/

these pictures are at 100x and 40 x.....

can someone please help me and tell me what it is......
thanks alot.

#2 rhombus

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Posted 15 March 2011 - 08:48 AM

hi guys we have recently had fungal contamination in our lab however it was thoroughly cleaned and all flasks changed etc etc.....today wehn i was doing regular viewing of my cells i saw something blurry. when i fine focused into the blur i observerd this.

http://img233.images...dsc00545cn.jpg/

http://img826.images.../dsc00538e.jpg/

http://img135.images.../dsc00541c.jpg/

these pictures are at 100x and 40 x.....

can someone please help me and tell me what it is......
thanks alot.



It looks like the bottom of the plastic flask/dish to me.....i.e. you have not visulaised the cells.

Kindest regards.

Uncle Rhombus.

#3 postdoc

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Posted 15 March 2011 - 01:36 PM

those are the leftover of liquid on the bottom of your culture dish, not comtamination.

#4 Sathyen

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Posted 15 March 2011 - 07:10 PM


hi guys we have recently had fungal contamination in our lab however it was thoroughly cleaned and all flasks changed etc etc.....today wehn i was doing regular viewing of my cells i saw something blurry. when i fine focused into the blur i observerd this.

http://img233.images...dsc00545cn.jpg/

http://img826.images.../dsc00538e.jpg/

http://img135.images.../dsc00541c.jpg/

these pictures are at 100x and 40 x.....

can someone please help me and tell me what it is......
thanks alot.



It looks like the bottom of the plastic flask/dish to me.....i.e. you have not visulaised the cells.

Kindest regards.

Uncle Rhombus.



i did visualize the cells...but i saw this in the background. i thought it was the flask too.....so i tried to look at a new flask but the new flask wasnt like that. rather it was clear.... the reason i thought it might have been a contamination was because in our microbiology lab where we were conducting sterility check of some media which was clear at first but after about a week (yesterday) i found plaquess that resembled this pattern. thus i got skeptical...

#5 Sathyen

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Posted 15 March 2011 - 07:59 PM

i did visualize the cells...but i saw this in the background. i thought it was the flask too.....so i tried to look at a new flask but the new flask wasnt like that. rather it was clear.... the reason i thought it might have been a contamination was because in our microbiology lab where we were conducting sterility check of some media which was clear at first but after about a week (yesterday) i found plaquess that resembled this pattern. thus i got skeptical...

Sathyen

#6 SciCell

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Posted 18 March 2011 - 03:59 PM

You have scraped the bottom of flask with your pipette tip and so this beautiful design. Ha ha. I suppose there is nothing to worry if your cell are ok.

i did visualize the cells...but i saw this in the background. i thought it was the flask too.....so i tried to look at a new flask but the new flask wasnt like that. rather it was clear.... the reason i thought it might have been a contamination was because in our microbiology lab where we were conducting sterility check of some media which was clear at first but after about a week (yesterday) i found plaquess that resembled this pattern. thus i got skeptical...

Sathyen



#7 Irfan A Khan

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Posted 21 June 2011 - 11:23 AM

looks so much like snow flakes!
could it be crystal of some salt?

#8 bob1

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Posted 21 June 2011 - 05:08 PM

Yep, salt crystals from something left to dry on the bottom of the flask. Nothing to worry about, other than it being an indicator that you probably spilled something and didn't clean it up.

#9 briguy7

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Posted 20 September 2011 - 08:42 AM

How festive!




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