Dear all,
I wonder if RNA (not cDNA) be amplified in 2-step qPCR? Or there is jus contamination! I am using KAPA™ SYBR® FAST qPCR Kit.
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tea-test
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Posted 15 March 2011 - 08:04 AM
tkmz, on 15 March 2011 - 05:38 AM, said:
Dear all,
I wonder if RNA (not cDNA) be amplified in 2-step qPCR? Or there is jus contamination! I am using KAPA™ SYBR® FAST qPCR Kit.
I wonder if RNA (not cDNA) be amplified in 2-step qPCR? Or there is jus contamination! I am using KAPA™ SYBR® FAST qPCR Kit.
you can possibly get signal from the genomic DNA but not from RNA.
tea-test: The artist formerly known as Ned Land
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tkmz
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Posted 15 March 2011 - 06:52 PM
So if I suspect there is a contamination in my cDNA after running NTC in the qPCR, I can rule out RNA contamination. Thanks!
