mutation using overlapping PCR, what's the success rate?
Posted 14 March 2011 - 09:24 PM
I was given a suggestion of using overlapping PCR to do a single nucleotide mutation, but some other people reckons that it's not going to work with a reason that the primers will mostly bind to the amplified portions and to get a full long product (overlapping)with a single nucleotide mutation in the middle of it will be almost impossible. Just wondering if anyone out there has any inputs with this method, and any suggestion with protocols that i can use?
thanks in advance!
Posted 15 March 2011 - 12:21 AM
Posted 28 June 2011 - 10:27 PM
Could you tell us your protocol?
didn't really work well for me, as I found out