resolving gel pourcentage
Posted 14 March 2011 - 03:49 PM
I want to run a gel SDS PAGe to do WB on my protein of 55kda.
I m wondering what could be better if do 10 or 12% gel acrylamide. since I would perform western blots of actin and my protein in the same membrane, but successively. without cutting the filter.
I already tried 10% gel, I saw my protein ,but after I couldnt separate well actin and my protein molecular weights, particulary for further quantification with image J.
thanks for advice
Posted 14 March 2011 - 10:51 PM
Posted 24 March 2011 - 07:47 AM
to separate better vgat from actin bands, 12% is good? since i found it in one paper!
Posted 24 March 2011 - 11:07 PM