Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

resolving gel pourcentage


  • Please log in to reply
3 replies to this topic

#1 jasmina

jasmina

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 134 posts
3
Neutral

Posted 14 March 2011 - 03:49 PM

Hi,
I want to run a gel SDS PAGe to do WB on my protein of 55kda.
I m wondering what could be better if do 10 or 12% gel acrylamide. since I would perform western blots of actin and my protein in the same membrane, but successively. without cutting the filter.
I already tried 10% gel, I saw my protein ,but after I couldnt separate well actin and my protein molecular weights, particulary for further quantification with image J.
thanks for advice

#2 protolder

protolder

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 293 posts
9
Neutral

Posted 14 March 2011 - 10:51 PM

Hola, I think can be better 8% than 12%, actin could run near the front but the resolution could be better. Buena suerte

#3 jasmina

jasmina

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 134 posts
3
Neutral

Posted 24 March 2011 - 07:47 AM

Hola, I think can be better 8% than 12%, actin could run near the front but the resolution could be better. Buena suerte


to separate better vgat from actin bands, 12% is good? since i found it in one paper!

#4 protolder

protolder

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 293 posts
9
Neutral

Posted 24 March 2011 - 11:07 PM


Hola, I think can be better 8% than 12%, actin could run near the front but the resolution could be better. Buena suerte


to separate better vgat from actin bands, 12% is good? since i found it in one paper!

Hola, as you know concentrated gels are suitable for separation of small proteins and diluted for big ones . probably you have the reason (Im sorry) but all the coloured mw markers have protein in your interval. Look for some markers in your lab or institution and run two gels to test. I repeat my excuses if I confuse you. Buena suerte




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.