Unpredictablity of PCR product
Posted 14 March 2011 - 06:48 AM
Any help would be appreciated.
Posted 14 March 2011 - 02:11 PM
Hey, vortexing is not a good idea, because it breaks DNA, centrifugation shouldn't be a problem...
Thank you. I was wondering if Plasmid DNA could also be affected. The reason I asked was the DNA I am using is 2-3 years old. Should I be extra careful and just flick it before adding to my PCR tube? Thank you.
Posted 14 March 2011 - 06:40 PM
Posted 16 March 2011 - 12:05 PM
A common problem is using too much DNA in your PCR reaction. Try reducing the amounts used 10x or 100x.
I was wondering how much is "too much" DNA and what are the effects of having too much? Thnaks!