Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Proteinase K stock solution


  • Please log in to reply
3 replies to this topic

#1 cardosopedro

cardosopedro

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 127 posts
2
Neutral

Posted 14 March 2011 - 02:51 AM

Hi.

I want to prepare a stock solution of proteinase K. Since my powder is not DNAse-free, I want to inactivate any contaminating DNAse without affecting proteinase K. How can I do this?

Thanks!
:)

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,616 posts
118
Excellent

Posted 15 March 2011 - 11:56 AM

proteinase k is fully active in 0.5% sds.
talent does what it can
genius does what it must
i do what i get paid to do

#3 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,268 posts
213
Excellent

Posted 15 March 2011 - 05:07 PM

And it is active at high concentrations of EDTA. Adding EDTA will chelate the Mg++ ions, a necessary co-factor to DNAse.

#4 cardosopedro

cardosopedro

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 127 posts
2
Neutral

Posted 16 March 2011 - 01:46 AM

Ok, thanks.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.