2 replies to this topic

[DilushanChandrajith]

hi everyone,
i have isolated total rna from an animal tissue and done the first strand synthesis (cDNA). but now i don't have isolated rna and i only have a very small amount of cDNA. so can i amplify that cDNA by PCR using random primers and using a lower annealing temperature?
THANK YOU!!!

[pcrman]

It is possible to amplify cDNA for expression analysis. You can read the following papers to know more

High-copy cDNA amplification of minimal total RNA quantities for gene expression analyses.
Simple method for cDNA amplification starting from small amount of total RNA.

[DilushanChandrajith]

pcrman, on 12 March 2011 - 11:19 PM, said:

It is possible to amplify cDNA for expression analysis. You can read the following papers to know more

High-copy cDNA amplification of minimal total RNA quantities for gene expression analyses.
Simple method for cDNA amplification starting from small amount of total RNA.

THANK YOU VERY MUCH,

I'm new to this subject......

Edited by DilushanChandrajith, 13 March 2011 - 02:31 AM.