What does low sequence complexity mean after bisulfite conversion?
0
2 replies to this topic
#2
pcrman
-
- Global Moderators
-
- 1,022 posts
Epigenetist
45
Excellent
Excellent
Posted 12 March 2011 - 11:13 PM
After bisulfite conversion, all unmethylated cytosines and non-CpG cytosines become uracil and then thymidine upon PCR, so the sequence is extremely cytosine depleted and contains many long stretches of thymidines, a feature known as low complexity.
