low sequence complexity
2 replies to this topic
Posted 12 March 2011 - 08:30 PM
What does low sequence complexity mean after bisulfite conversion?
Posted 12 March 2011 - 11:13 PM
After bisulfite conversion, all unmethylated cytosines and non-CpG cytosines become uracil and then thymidine upon PCR, so the sequence is extremely cytosine depleted and contains many long stretches of thymidines, a feature known as low complexity.