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low sequence complexity


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#1 Boba

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Posted 12 March 2011 - 08:30 PM

What does low sequence complexity mean after bisulfite conversion?

#2 pcrman

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Posted 12 March 2011 - 11:13 PM

After bisulfite conversion, all unmethylated cytosines and non-CpG cytosines become uracil and then thymidine upon PCR, so the sequence is extremely cytosine depleted and contains many long stretches of thymidines, a feature known as low complexity.

#3 Boba

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Posted 13 March 2011 - 09:06 AM

thank you!




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