5 replies to this topic

[jaknight]

I have an unstained high-molecular weight protein ladder and I would like to add a dye so that I can see the bands during electrophoresis.

Does anyone know a dye I can add to the ladder to do this? I'm thinking ponceau maybe.

[K.B.]

I think those colourful protein ladders use conjugated dyes, not just added freely.

[bob1]

Yep, rainbow marker etc all have conjugated bands, I don't know of any marker that can be added and will stay with the protein during electrophoresis.  YOu could however make a loading dye with different size dyes (e.g. bromophenol blue, orange G, xyxlene cyanol...) to act as a proxy for the ladder.

[madrius1]

How about coomassie staining? If it stains protein in-gel, it should stain them in solution..

[mdfenko]

madrius1, on 14 March 2011 - 09:09 AM, said:

How about coomassie staining? If it stains protein in-gel, it should stain them in solution..

coomassie can be removed electrophoretically. it won't stay with the protein during electrophoresis (yes, i have seen the dye come off during electrophoresis of coomassie stained samples).

[madrius1]

Didn't know that ^^