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dye protein ladder


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5 replies to this topic

#1 jaknight

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Posted 11 March 2011 - 11:06 AM

I have an unstained high-molecular weight protein ladder and I would like to add a dye so that I can see the bands during electrophoresis.

Does anyone know a dye I can add to the ladder to do this? I'm thinking ponceau maybe.

#2 K.B.

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Posted 11 March 2011 - 12:01 PM

I think those colourful protein ladders use conjugated dyes, not just added freely.

#3 bob1

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Posted 13 March 2011 - 12:26 PM

Yep, rainbow marker etc all have conjugated bands, I don't know of any marker that can be added and will stay with the protein during electrophoresis. YOu could however make a loading dye with different size dyes (e.g. bromophenol blue, orange G, xyxlene cyanol...) to act as a proxy for the ladder.

#4 madrius1

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Posted 14 March 2011 - 09:09 AM

How about coomassie staining? If it stains protein in-gel, it should stain them in solution..

#5 mdfenko

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Posted 15 March 2011 - 12:50 PM

How about coomassie staining? If it stains protein in-gel, it should stain them in solution..


coomassie can be removed electrophoretically. it won't stay with the protein during electrophoresis (yes, i have seen the dye come off during electrophoresis of coomassie stained samples).
talent does what it can
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#6 madrius1

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Posted 16 March 2011 - 10:13 AM

Didn't know that ^^




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