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protocol to transfer protein on transfer membrane fluoride bind


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4 replies to this topic

#1 drgajananbhole

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Posted 10 March 2011 - 07:47 AM

please tell me how to transfer proteins from sds page to transfer membrane fluoride bind ( serva) with help of semi dry blotting equipment

#2 mdfenko

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Posted 10 March 2011 - 12:03 PM

do you have the manual that came with the semi-dry transfer unit? different brands have different recommended methods.

in the meantime, read this handbook:

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#3 drgajananbhole

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Posted 11 March 2011 - 12:03 PM

@thank u very much for reply we have a semi dry blotting unit from "cleaver scientific pt ltd" plz tell me if u have any idea about it & also the composition of transfer buffer. mainly the conc. of methanol

#4 Ihsan

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Posted 12 March 2011 - 03:09 AM

do you have the manual that came with the semi-dry transfer unit? different brands have different recommended methods.

in the meantime, read this handbook:


Hi, I tried to open the pdf but i couldn't.

Can u email it to my address ihsanahmad@hotmail.co.uk

Thanks

#5 mdfenko

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Posted 15 March 2011 - 12:28 PM

i can't access cleaver's website. if you can then you should be able to download the manual for your semi-dry unit. that will give you the information that you need.

usually methanol is 10-20% in the transfer buffer.

talent does what it can
genius does what it must
i do what i get paid to do




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