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Dissolved Primer pellet in pure ethanol instead of water


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#1 Moebius

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Posted 09 March 2011 - 06:53 AM

I accidentally grabbed the ethanol bottle instead of the water one. The ethanol bottle has 100% ethanol. I noticed the stock solution didn't freeze at -20 C and I didn't get any results for my PCR reaction. I was wondering if I can just pipette out the ethanol and add the appropriate amount of water into the primer tubes. I can still see the pellet, but some of the pellet has gotten bigger in area. I really appreciate any help in advance! Thank you!

#2 philman

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Posted 18 March 2011 - 06:37 AM

you should be able to, some protocols require DNA precipitation in 100% ethanol anyway. I'd spin the tube down again to make sure the pellet is whole, then pipette off as much of the ethanol without disturbing the pellet as you can, then just leave to air dry before re-suspending in water or buffer.




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