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Dissolved Primer pellet in pure ethanol instead of water

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#1 Moebius



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Posted 09 March 2011 - 06:52 AM

I accidentally grabbed the ethanol bottle instead of the water one. The ethanol bottle has 100% ethanol. I noticed the stock solution didn't freeze at -20 C and I didn't get any results for my PCR reaction. I was wondering if I can just pipette out the ethanol and add the appropriate amount of water into the primer tubes. I can still see the pellet, but some of the pellet has gotten bigger in area. I really appreciate any help in advance! Thank you!

#2 mdfenko


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Posted 09 March 2011 - 12:25 PM

why don't you let the ethanol evaporate then resuspend the pellet in water. that way you shouldn't lose any.

you can speed up the evaporation with a speedvac or n-evap.

Edited by mdfenko, 09 March 2011 - 12:26 PM.

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