Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Dissolved Primer pellet in pure ethanol instead of water


  • Please log in to reply
1 reply to this topic

#1 Moebius

Moebius

    member

  • Active Members
  • Pip
  • 14 posts
0
Neutral

Posted 09 March 2011 - 06:52 AM

I accidentally grabbed the ethanol bottle instead of the water one. The ethanol bottle has 100% ethanol. I noticed the stock solution didn't freeze at -20 C and I didn't get any results for my PCR reaction. I was wondering if I can just pipette out the ethanol and add the appropriate amount of water into the primer tubes. I can still see the pellet, but some of the pellet has gotten bigger in area. I really appreciate any help in advance! Thank you!

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,781 posts
132
Excellent

Posted 09 March 2011 - 12:25 PM

why don't you let the ethanol evaporate then resuspend the pellet in water. that way you shouldn't lose any.

you can speed up the evaporation with a speedvac or n-evap.

Edited by mdfenko, 09 March 2011 - 12:26 PM.

talent does what it can
genius does what it must
i do what i get paid to do




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.