Dissolved Primer pellet in pure ethanol instead of water
Posted 09 March 2011 - 06:51 AM
Posted 09 March 2011 - 09:48 PM
Call up the company. See, if they have any suggestions to troubleshoot this.
Technically, you have lost the primers. So, it wouldn't hurt to try this. If you can see the pellet, pipette out ethanol, dry the vial completely , dissolve the pellet in water or TE and see what happens
Note: This is just a suggestion and there is no guarantee it will work. Try at your own risk
Post results, whatever the outcome.
All the best
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Posted 10 March 2011 - 12:03 AM
You used the primers (in the ethanol) in a pcr reaction , a normal one? Or?
That could be due so many factors.
Just try to evaporate the ethanol, spin down the vial, and let the ethanol evaporate.
And you could indeed try to pipette out a bit of the top of the ethanol, but in order not to risk anything: let it evaporate for the bigest part.