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NuPAGE Gel get smaller after staining o/n


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#1 saad

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Posted 08 March 2011 - 09:45 PM

Hi All

I ran a NuPAGE 4-12% Bis Tris for protein. I stained overnight with Coomassie stain and we use this stain before and after and everythibg is allright. Next morning when I want to destain I saw the gel get smaller than it sholud be but I see the bands on the gel. My question has it happened to anyone to see something similar to that.

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#2 mdfenko

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Posted 09 March 2011 - 01:12 PM

methanol in the staining solution caused the gel to shrink. the destaining solution should allow the gel to swell back to the original size if there is a lower concentration of methanol (we use 50% methanol in the stain and 30% in the destain).


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