Hi All
I ran a NuPAGE 4-12% Bis Tris for protein. I stained overnight with Coomassie stain and we use this stain before and after and everythibg is allright. Next morning when I want to destain I saw the gel get smaller than it sholud be but I see the bands on the gel. My question has it happened to anyone to see something similar to that.
Regards
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1 reply to this topic
#2
mdfenko
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an elder
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Excellent
Excellent
Posted 09 March 2011 - 01:12 PM
methanol in the staining solution caused the gel to shrink. the destaining solution should allow the gel to swell back to the original size if there is a lower concentration of methanol (we use 50% methanol in the stain and 30% in the destain).
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genius does what it must
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