Hi All
I ran a NuPAGE 4-12% Bis Tris for protein. I stained overnight with Coomassie stain and we use this stain before and after and everythibg is allright. Next morning when I want to destain I saw the gel get smaller than it sholud be but I see the bands on the gel. My question has it happened to anyone to see something similar to that.
Regards
Submit your paper to J Biol Methods today!
1 reply to this topic
#2
mdfenko
-
- Active Members
-
- 2,848 posts
an elder
145
Excellent
Excellent
Posted 09 March 2011 - 01:12 PM
methanol in the staining solution caused the gel to shrink. the destaining solution should allow the gel to swell back to the original size if there is a lower concentration of methanol (we use 50% methanol in the stain and 30% in the destain).
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
