1 reply to this topic

[saad]

Hi All

I ran a NuPAGE 4-12%  Bis Tris  for protein. I stained overnight with Coomassie stain and we use this stain before and after and everythibg is allright. Next morning when I want to destain I saw the gel get smaller than it sholud be but I see the bands on the gel. My question has it happened to anyone to see something similar to that.

Regards

[mdfenko]

methanol in the staining solution caused the gel to shrink. the destaining solution should allow the gel to swell back to the original size if there is a lower concentration of methanol (we use 50% methanol in the stain and 30% in the destain).