Hello,
I am trying to seed some flasks with some Min6 cells obtained from a frozen stock. I dont have a standard protocol for this as of yet and hence am using the protocols I find in papers.
Does anyone has any idea
1)how long it takes for this cell line to come out of "shock' as I have been working with then for eight days and have only been changing media.
2)Tried splitting one of my flasks today but soemhow felt the trypsinisation time of 1 minutes of 0.25&trypsin-EDTA wasnt able to detach the cells.
3) After 1 minute of the trypsin addition, I removed the trypsin and gave the flasks a "knock'/bang to displace the cells before put in some more media. i am wondering if I should incubate the cells for a few minutes at 37oC before I do this.
Please share your thoughts if you have worked with this cell line before
Thanks.
Vinder
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