Hi, I have problem with my tris/tricine sds page gel recently. I have been using it for a couple of years. I didn't run gels for a while. When I started to run 2 weeks ago, I found got this problem. The dye front of the samples is diffused and uneven (not forming a straight line) and the dye smeared very badly. The protein ladder runs nicely, focused well. The Dye smear interfered the low molecular weight peptides.
The gel is a standard 15% tris-tricine sds page. I run it in the cold room.
It is very similar to the problem described here "Trouble-shooting Tris-Tricine SDS-PAGE http://www.protocol-...sts/41821.html"
I'll really appreciate your advice.
Edited by ly07, 08 March 2011 - 01:22 PM.