During nucleic acid extraction using column, the RNA/DNA bind to the membrane and in the last step, eluted by buffer/water? What is in the buffer/water OR what is not in the buffer/water that causes the DNA/RNA from being released from the membrane?
0
1 reply to this topic
#2
Maddie
-
- Active Members
-
- 289 posts
Veteran
2
Neutral
Neutral
Posted 10 March 2011 - 08:38 AM
hianghao, on 07 March 2011 - 09:42 PM, said:
During nucleic acid extraction using column, the RNA/DNA bind to the membrane and in the last step, eluted by buffer/water? What is in the buffer/water OR what is not in the buffer/water that causes the DNA/RNA from being released from the membrane?
It has to do with salt and pH. For the DNA to bind to silica you need lots of salt and a low pH (below 7.5). To release the DNA you elute with a solution that is poor in salt and has a pH >7.5 (the EB in the Qiagen kit is at pH 8.0 I think).
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.
A. Einstein
A. Einstein
