Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

FBS recommendation for HEK293T cells


  • Please log in to reply
5 replies to this topic

#1 lora

lora

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 07 March 2011 - 07:50 AM

dear all,
i just have ordered HEK293T cells from openbiosystems, Catalog number: HCL4517

in the manual they recommend certain FBS type (Thermo Scientific Hyclone No.: 30014.03), but the minimum amount that can be ordered is 100l and i will never use it off, since we actually just plan to include the cells in our cell line library. My question is: have you experience with this type of cells? could you recommend another lot of FBS? or do you know how to get a trying exemplar of the recommended one?

thank a lot for any help

#2 Rsm

Rsm

    Post Dog

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 381 posts
8
Neutral

Posted 07 March 2011 - 11:40 PM

HEK293T are not very sensitive to the kind/batch of FBS that you use. We even use NCS, that works fine as well, the cells still grow very well. Just use your usual serum should do it.
I got soul, but I'm not a soldier

#3 lora

lora

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 08 March 2011 - 01:22 AM

thanks a lot that will simplify my life a bit:-)

#4 Rsm

Rsm

    Post Dog

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 381 posts
8
Neutral

Posted 08 March 2011 - 07:14 AM

It's also not unusual to test serum batches before you buy them. That is, you can ask them to provide you a "sample" of that particular serum. So you can try it (compare to your normal serum). And then you suddenly realize that it's not good at all, and don't have buy the 100l.
:)
I got soul, but I'm not a soldier

#5 lora

lora

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 09 March 2011 - 12:06 AM

yeah i had this idea too :rolleyes: and ordered one yesterday

#6 damon

damon

    member

  • Active Members
  • Pip
  • 14 posts
0
Neutral

Posted 22 March 2011 - 12:38 AM

sounds like a convincing alternative

yeah i had this idea too :rolleyes: and ordered one yesterday






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.