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Isothermal target mRNA amplification on solid state?


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#1 ttrndy

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Posted 06 March 2011 - 10:08 PM

Hi Everyone

I知 currently work on cancer cell mutation analysis. I知 going to amplify the mRNA for further research. I need an amplification techniques that can amplify mRNA in iso-thermal condition,primer attached on the solid surface. Are there anyone else know about that techniques?

I also take a look at RCA and RAM but it seems unsatify my purpose because i need to amplify the mRNA not amplify the signal. I hope to receive your advices soon.

My email: tranduy.bio@gmail.com

All the best





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