Posted 06 March 2011 - 02:38 AM
if not, how can I make sure the primers remain on the template for about an hour?
this is not a theoretical question, my experiment depends on the answer.
Posted 06 March 2011 - 03:49 AM
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that did belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 06 March 2011 - 04:38 AM
follow up question: what if after the one hour at room temperature I need to release the primers from the plasmid? I guess if I just heat it to 95 and cool it again, the primers will get back on the plasmid. but I don't want the primers anymore at that point. is there a way to do that?