Can Qiagen columns remove genomic DNA?
Posted 04 March 2011 - 10:47 PM
Earlier this evening, I got into a debate with a labmate of mine. We were discussing the merits of column based DNA purification method versus modified alkaline lysis method for BAC extraction.
While there were many point which we disagreed on, one point she raised had me thinking.
She claimed that column based BAC purification has less contaminating bacterial genomic DNA. The reasoning being that genomic DNA being longer than BAC DNA, has a stronger affinity to the column matrix. Thus when the DNA is eluted from the column, BAC DNA is preferentially eluted.
I on the other hand think that at 10kb and larger the binding affinity of a BAC and sheered genomic DNA would be no different. Both would bind and dissociate with equal efficiency to the column matrix.
What is really happening within the column?
This question could be answered by experiment. But that would take actual work and time - next week.
Posted 05 March 2011 - 11:06 PM
Posted 13 March 2011 - 12:08 PM
Posted 16 March 2011 - 12:27 AM
Posted 28 March 2011 - 03:38 AM
I was just wondering where to post my question and I saw your discussion.
I work with RNA`s. Can you explain me how actually the step with Qiagen gDNA Eliminator spin column removes the gDNA from the sample? It is based on.....something like size-excluzion filtration, or...?
Posted 28 March 2011 - 02:47 PM
Posted 02 April 2011 - 09:43 PM