3 replies to this topic

[atorbidoni]

Guys, I am trying to do MTT assay on cells growing in suspention. I have to incubate with a drug and then to applied the MTT, I do not have a centrifuge of 96-well plate. Somebody have some protocol to do MTT on suspention cells? Thanks

Ana

[Neurite]

The only thing I could think of, was, you can collect all the well content and place it in small eppendorff tubes so you can centrifugate them.... I think the formazan crystals/cells would form a pellet... you can discard all the media and then add the isopropanol, DMSO or whatever you use to dissolve your crystals...

It sounds tedious though...

[JRK]

Have you tried XTT? If you want it , I can  send you the protocol

[PostDocTrauma]

we're going to try and use alamar blue for our suspension cells.

we found the suspension cells do not stick to the plate even after we spin them down