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MTT with cells growhing in suspension, not adherent


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3 replies to this topic

#1 atorbidoni

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Posted 03 March 2011 - 02:18 PM

Guys, I am trying to do MTT assay on cells growing in suspention. I have to incubate with a drug and then to applied the MTT, I do not have a centrifuge of 96-well plate. Somebody have some protocol to do MTT on suspention cells? Thanks

Ana

#2 Neurite

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Posted 04 March 2011 - 07:04 PM

The only thing I could think of, was, you can collect all the well content and place it in small eppendorff tubes so you can centrifugate them.... I think the formazan crystals/cells would form a pellet... you can discard all the media and then add the isopropanol, DMSO or whatever you use to dissolve your crystals...

It sounds tedious though...

#3 JRK

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Posted 02 April 2011 - 12:37 AM

Have you tried XTT? If you want it , I can send you the protocol

#4 PostDocTrauma

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Posted 03 April 2011 - 11:37 PM

we're going to try and use alamar blue for our suspension cells.

we found the suspension cells do not stick to the plate even after we spin them down




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