2 rounds PCR got problem
Posted 03 March 2011 - 09:30 AM
I always got a good product after 1st round of PCR and used it as the template for the 2nd round but could never get good result.
Would it be the problem with my primers? Because I used same reverse primer for both 2 rounds of PCR.
Posted 03 March 2011 - 09:37 AM
Posted 03 March 2011 - 01:12 PM
Thank you. I designed an extra 'GCG' at both ends. Is it enough? I cannot put too many extra base pairs because I already had some extra uncomplimentary base pairs to introduce my restriction sites and tag.