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Acrylamide gel load capacity

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#1 mcb56



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Posted 03 March 2011 - 07:27 AM

Hello, I would like to know how much RNA I can put on an acrylamide gel. I am going to be running a 8% gel, 1mm thick with wells about 10cm long. I would ideally like to get around 100ug of RNA on the gel. Are there any general guidelines for determining gel load capacity?

Thank You

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