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How to block ProteinG-Sepharose Beads

Started by mcan6, Mar 02 2011 10:24 PM

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#1 mcan6

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Posted 02 March 2011 - 10:24 PM

Dear All,
For ChIP assay, how do you guys block the beads? I am using Gammabind G Sepharose.

Thanks in advance

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#2 chabraha

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Posted 06 March 2011 - 02:22 PM

5% BSA & 10ug/mL sheared Salmon Sperm DNA in Dilution Buffer would be my suggestion.

Treasure Chest Wizardry

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