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Stripping Antibodies on Immunohistochemistry


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#1 11235

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Posted 02 March 2011 - 06:30 PM

Hi everyone,

I'm in the middle of doing an immunohistochemistry with mouse brain slices. I've already treated them with antibodies, but I just found out that the dilutions were wrong. The antibodies won't stain the slices because it's too weak. Is there anyway I can strip the antibodies? and then replace them with the properly diluted antibodies?

Thanks!

#2 Chelo

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Posted 02 March 2011 - 11:44 PM

If you simply used a too diluted antibody, there is no need to strip. I would simply reincubate the samples with the correct dilution.

#3 BioMiha

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Posted 03 March 2011 - 12:36 AM

I agree with Chelo. Stripping is notoriously difficult to achieve properly and if you just reincubate with the proper dilutions there will be absolutely no harm done.

#4 11235

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Posted 01 April 2011 - 01:13 PM

thanks! so much!




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