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GFP transfection using lipofectin


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#1 anonymous

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Posted 01 November 2001 - 10:00 PM

I'd like to find out the conditions required to sucessfully transiently transfect HepG2 cells with a mammalian expression plasmid containing the GFP protein using lipofectin,:The amount of plasmid requiredThe amount of time required for the plasmid to be incubated on the cellsThe amount of lipofectin requiredThank you!

#2 anthrobson

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Posted 30 April 2002 - 03:18 AM

Normally, you would have to optimise the experiment for conditions like that. Try LifeTech's website www.lifetech.com for a massive pdf file containing everything you could know about transfections and optimisations for new cell lines.

Basically, you use a 24-well plate with different DNA concentrations down in each row and different lipofectin concentrations along each row...

Good luck!





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